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DS Weinberg, GS Pinkus and KA Ault
A sensitive cytofluorometric technique, the "kappa-lambda test," permits
detection of small numbers of monoclonal B lymphocytes (clonal excess).
Such a method might represent a new diagnostic tool for diagnosis of
non-Hodgkin's lymphomas, potentially providing definitive evidence of
lymphomatous involvement in cases equivocal by standard immunologic
methods. To determine the significance of detecting B cell clonal excess in
lymphoid tissues, we applied the kappa-lambda test to cell suspensions from
60 consecutive specimens suspected to involvement by malignant lymphoma.
Results were correlated with the pathologic diagnosis and with standard
cell marker studies in each case. B cell clonal excess was observed in 24
of the 25 cases of non-Hodgkin's lymphoma of B cell origin, including a
single case involving early detection of recurrence. None of the remaining
cases, including benign reactive hyperplasia, T cell lymphoma, and
Hodgkin's disease, showed evidence of B cell clonal excess. Selective
examination of cell subpopulations was also achieved using this
cytofluorometric method. We conclude that the detection of B cell clonal
excess by the kappa-lambda test represents a new approach to the diagnosis
of B cell lymphoma, which provides certain advantages over more standard
methods of cell marker analysis.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
