Glutathione-dependent protection against oxidative damage of the human red
cell membrane
S Fujii, GL Dale and E Beutler
Glutathione (GSH) dependent protection against oxidative damage of human
red cell membrane was examined. An artificial system was used in which
chloroform/methanol-extracted red cell lipids, in the form of liposomes,
were subjected to attack by a peroxidation system consisting of
ascorbate-Fe3+. Human erythrocytes contained a nondialyzable factor,
completely inactivated by heating in a boiling water bath for 3 min, which
showed GSH-dependent inhibition against lipid peroxidation and was devoid
of GSH peroxidase activity. On the other hand, GSH-S transferase, highly
purified by affinity chromatography, had no inhibitory activity. These
findings strongly indicate that the GSH- dependent protection against lipid
peroxidation of human red cell membrane is mediated by one or more proteins
other than GSH peroxidase and GSH-S transferase.
Volume 63,
Issue 5,
pp. 1096-1101,
05/01/1984
Copyright © 1984 by The American Society of Hematology
