In vitro cytodestruction of human leukemic cells using murine monoclonal
antibodies and human complement
DE Stepan, RM Bartholomew and TW LeBien
We have investigated the ability of murine monoclonal antibodies (MoAb) to
lyse human leukemic cells in vitro using human serum as a source of
complement (C'). The human C'-fixing ability of five of seven MoAb is
documented. Studies with two of these MoAb (BA-1 and BA-2) indicated that
their human C'-fixing ability and subsequent lysis of leukemic cells was
through activation of the classical pathway of C', was independent of donor
serum source, and occurred with a number of different target cells. BA-1
and BA-2 could effectively lyse fresh leukemic cells in the presence of
100% human serum, and BA-1 plus human serum could effectively lyse leukemic
cells in the presence of a 20- fold excess of normal human bone marrow
cells. Our results have potential implications for immunotherapy trials
utilizing murine MoAb.
Volume 63,
Issue 5,
pp. 1120-1124,
05/01/1984
Copyright © 1984 by The American Society of Hematology
