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R Pennathur-Das, E Alpen, E Vichinsky, J Garcia and B Lubin
To investigate the cellular events that accompany erythroid hyperplasia, we
studied several effects of erythropoietin (Epo) on marrow CFU-E in sickle
cell anemia (SCA). We measured CFU-E number, CFU- E growth as a function
both of Epo exposure time and of Epo concentration, and suppression of
Epo-induced CFU-E formation by anti- Epo antiserum. With 0.5 U Epo/ml, the
number of CFU-E was elevated in SCA (1,087 +/- 520) compared to normal (430
+/- 130). CFU-E were formed even when Epo was immediately neutralized by a
1/150 dilution of anti- Epo. After 40 hr of Epo exposure, only 2% of total
CFU-E were expressed in normal marrow, whereas 12%-40% of CFU-E were
expressed in SCA. Inhibition of CFU-E growth required at least 1/50
dilution of anti-Epo in SCA and a 1/300 dilution in normal marrow. In
contrast to normal, a small number (5%-20%) of CFU-E were expressed in the
absence of added Epo in SCA, and this pool required a 1/150 dilution of
anti-Epo for inhibition. The Epo dose-response curve in SCA revealed a peak
in colony formation around 0.1 U Epo/ml and 0.5 U Epo/ml, whereas only one
peak at 0.5 U Epo/ml was seen in normals. These data strongly suggest that,
in response to the demands of chronic erythroid hyperplasia in SCA, a pool
of CFU-E is present characterized by increased in vitro sensitivity to Epo.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
