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Human platelet fibrinogen gamma chain structure

MW Mosesson, GA Homandberg and DL Amrani

Human plasma fibrinogen is produced by liver parenchymal cells. Such molecules contain two classes of gamma-chains (gamma A, gamma'), which differ with respect to their COOH-terminal sequences. When fibrin is crosslinked in the presence of factor XIIIa and Ca2+, three types of gamma-dimer are formed (gamma A-gamma A; gamma A-gamma'; gamma'- gamma'). A separate fibrinogen pool is located in platelet alpha- granules. We analyzed this fibrinogen to determine whether gamma'- chains were present to the same extent (7%) that they are found in plasma fibrinogen. Electrophoretic analysis (Laemmli system) of reduced samples of the clot that formed subsequent to release of fibrinogen from thrombin-stimulated washed platelets, revealed a single crosslinked gamma-dimer band in the gamma A-gamma A position. Material collected into EDTA-containing buffer and subsequently crosslinked in the presence of added factor XIII and Ca2+ also revealed a gamma A- gamma A dimer band. This finding was further investigated by Western blotting of reduced gel specimens that had been reacted with an anti- gamma-chain antibody followed by 125I-labeled protein A. A single type of gamma-chain, gamma A, was present in the fibrinogen from a Triton X- 100 or 10 M urea platelet lysate, or in noncrosslinked fibrin obtained from thrombin-treated platelets. Crosslinked reduced fibrin from thrombin-treated platelets or that prepared from the Triton lysate revealed a single type of gamma-dimer, gamma A-gamma A. We conclude that there are no gamma'-chains (less than 1%) in platelet fibrinogen. This structural difference from hepatic fibrinogen probably results from differences in the processing and/or regulation of the fibrinogen gamma-chain gene in megakaryocytes.

Volume 63, Issue 5, pp. 990-995, 05/01/1984
Copyright © 1984 by The American Society of Hematology


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S. Uitte de Willige, K. F. Standeven, H. Philippou, and R. A. S. Ariens
The pleiotropic role of the fibrinogen {gamma}' chain in hemostasis
Blood, November 5, 2009; 114(19): 3994 - 4001.
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  Copyright © 1984 by American Society of Hematology         Online ISSN: 1528-0020