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Factor VIII-von Willebrand factor requires calcium for facilitation of
platelet adherence
KS Sakariassen, M Ottenhof-Rovers and JJ Sixma
The role of divalent cations in platelet adherence to deendothelialized
human arteries in flowing blood was investigated in an annular perfusion
chamber. Spreading of platelets on the subendothelium was impaired below 30
microM of free Ca2+ ions (Ca2+). When Ca2+ was replaced by Mg2+, adherence
was unchanged in perfusates without exogenous factor VIII-von Willebrand
factor (FVIII-vWF), but the ability of FVIII-vWF to support platelet
adherence was lost. Binding of FVIII-vWF to the vessel wall was independent
of divalent cations, but bound FVIII-vWF was only able to mediate adherence
after exposure to Ca2+. Pretreatment of FVIII-vWF with the calcium chelator
EGTA (10 mM) resulted in loss of the ability to facilitate platelet
adherence, while the ristocetin cofactor activity remained intact. Full
restoration of the ability to mediate platelet adherence could only be
obtained by prolonged dialysis against Ca2+ in the millimolar range. These
data indicate that divalent cations have at least two separate roles to
play in supporting platelet adherence: (1) platelet spreading on the
subendothelium requires Ca2+ or Mg2+; (2) FVIII-vWF should be exposed to
Ca2+ to obtain its optimal biologic activity in supporting platelet
adherence.
Volume 63,
Issue 5,
pp. 996-103,
05/01/1984
Copyright © 1984 by The American Society of Hematology
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