Analysis of myelomonocytic leukemic differentiation by a cell surface
marker panel including a fucose-binding lectin from Lotus tetragonolobus
L Elias and DE Van Epps
The fucose-binding lectin from Lotus tetragonolobus ( FBL -L) has been
previously shown to bind specifically to normal cells of the myeloid and
monocytic lineages. The purpose of this study was to explore the utility of
fluoresceinated FBL -L as a leukemia differentiation marker in conjunction
with a panel of other frequently used surface markers (Fc receptor, HLA-DR,
OKM1, and antimonocyte antibody). FBL -L reacted with leukemic cells in 8/9
cases of clinically recognized acute myeloid leukemia, including myeloid
blast crisis of chronic granulocytic leukemia, 3/3 cases of chronic phase
chronic myelogenous leukemia, and in 2/7 cases of clinically
undifferentiated acute leukemia. Correlations were noted between reactivity
with FBL -L, and DR and Fc receptor expression. Among continuous cell
lines, FBL -L bound with high intensity to a majority of HL-60 and U937
cells. The less well differentiated myeloblast cell lines, KG-1, KG1a , and
HL-60 blast II, exhibited less FBL -L binding than HL-60 and U937. A
moderate proportion of K562 cells exhibited low level binding of FBL -L.
Several lymphoblastic cell lines exhibited a pattern of low intensity
binding that was distinguishable from the high intensity binding pattern of
the myeloblastic lines. FBL -L reactivity of U937 was enhanced by induction
of differentiation with leukocyte conditioned medium, but not
dimethylsulfoxide. Such treatments induced contrasting patterns of change
of HL-60 and U937 when labeled with OKM1, alpha-Mono, and HLA- DR. These
studies demonstrate the application of FBL -L to analysis and quantitation
of myelomonocytic leukemic differentiation.
Volume 63,
Issue 6,
pp. 1285-1290,
06/01/1984
Copyright © 1984 by The American Society of Hematology
