The effect of prostaglandins E1 and E2 on macrophage progenitor cells with
high proliferative potential in mouse bone marrow in vitro
AB Kriegler, TR Bradley and GS Hodgson
High proliferative potential macrophage progenitor cells (HPP-CFC) in 5-
fluorouracil (FU) treated and normal mouse bone marrow (BM) have been shown
to be less sensitive to inhibition of proliferation by prostaglandins of
the E series (PGE) than low proliferative potential macrophage progenitor
cells (LPP-CFC) in normal BM in agar cultures. The growth of large colonies
(diameter greater than 0.5 mm) derived from HPP-CFC in FU BM, which require
a combination of macrophage colony- stimulating factor (CSF-1) plus a new
growth factor called synergistic activity (SA), are inhibited by 50% in the
presence of 5.5 X 10(-6) M PGE1. On the other hand, LPP-CFC in normal BM,
which form smaller colonies (diameter less than or equal to 0.5 mm) in the
presence of CSF- 1 alone, require only 5 X 10(-8) M PGE1 for the same level
of inhibition. Addition of appropriate concentrations of PGE1 to the agar
culture assay should improve detection of HPP-CFC by inhibiting the
proliferation of LPP-CFC. These observations suggest that the apparent
negative feedback control of macrophage production by PGE operates largely
on the LPP-CFC, which respond to CSF-1 alone, and is probably not involved
in the regulation of the more primitive HPP-CFC.
Volume 63,
Issue 6,
pp. 1348-1352,
06/01/1984
Copyright © 1984 by The American Society of Hematology
