Two-dimensional membrane protein patterns of acute myeloid leukemia cells
and mature myeloid cells after various ectolabeling procedures
JG de Jong, AW Dekker, R Kapteijn and JJ Sixma
Surface exposed membrane proteins of malignant cells may offer important
clues about the differentiation stage of the cell or may contain proteins
specific for the malignant state. We have studied the surface exposed
membrane proteins of human acute myeloid leukemia cells employing the
lactoperoxidase, periodate, or the neuraminidase/galactose oxidase
ectolabeling procedures. One- dimensional membrane protein patterns were
prepared from 20 patients, and from 19 patients, two-dimensional patterns
were prepared according to O'Farrell. No consistent differences in membrane
proteins could be found between patients classified as M1, M2, M4, or M5
(FAB classification). A diagram of membrane proteins from acute myeloid
leukemia cells subjected to two-dimensional electrophoresis could be
composed from the results obtained. About 25 different membrane proteins
can be indicated. Two-dimensional patterns, after the various ectolabeling
procedures, were also prepared from mature myeloid cells, visualizing about
18 different membrane proteins. Comparison of these and the
undifferentiated myeloid leukemia cell pattern reveals some
maturation-linked or leukemia-associated differences. The most relevant
proteins will be discussed, along with their association with a recently
described "malignancy marker" with a molecular weight of 68,000 daltons.
Volume 64,
Issue 1,
pp. 110-122,
07/01/1984
Copyright © 1984 by The American Society of Hematology
