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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bai, Y. Articles by Hogg, N. Search for Related Content PubMed PubMed Citation Articles by Bai, Y. Articles by Hogg, N. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Monoclonal antibodies specific for platelet glycoproteins react with human monocytes Y Bai, H Durbin and N Hogg Three monoclonal antibodies, P256 , P140, and P112 , react with the 135,000 mol wt IIb component of the glycoprotein IIb/IIa complex. They also react with a 200,000-mol wt protein present at low levels in the complex. Using immunofluorescence techniques, monoclonal antibodies P140 and P112 , but not P256 , can be shown to bind to 80% of human monocytes. However, P256 was able to immunoprecipitate the IIb/IIIa complex from detergent-solubilized monocytes, suggesting that the P256 epitope is less accessible on monocytes than on platelets. The monoclonal antibodies also precipitated molecules of approximately 200,000 mol wt from the monocytes. Two other monoclonal antibodies, J15 (specific for the IIb/IIIa complex) and AN51 (which reacts with the second major platelet glycoprotein complex, I), also react with monocytes. Binding of the monoclonal antibodies to the histiocytic cell line, U937, and promyelocytic cell line, HL-60, reflected the pattern of reaction with monocytes. The presence on monocytes of these glycoproteins, instrumental to the role of platelets in clotting, raises the possibility that monocytes might have similar functions in particular circumstances. Volume 64, Issue 1, pp. 139-146, 07/01/1984 Copyright © 1984 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Tropel, V. Roullot, M. Vernet, C. Poujol, H. Pointu, P. Nurden, G. Marguerie, and D. Tronik-Le Roux A 2.7-kb Portion of the 5' Flanking Region of the Murine Glycoprotein alpha IIb Gene Is Transcriptionally Active in Primitive Hematopoietic Progenitor Cells Blood, October 15, 1997; 90(8): 2995 - 3004. [Abstract] [Full Text] [PDF]

	Copyright © 1984 by American Society of Hematology         Online ISSN: 1528-0020