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A cell line secreting stimulating factors for CFU-GEMM culture
CD Myers, FE Katz, G Joshi and JL Millar
The multipotent hemopoietic stem cell has fastidious growth requirements in
vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned
medium has been used to supply the undefined growth factors required for
culture of the human multipotent hemopoietic progenitor. We describe the
use of medium conditioned by the bladder carcinoma cell line, 5637, to
replace PHA-LCM in CFU-GEMM cultures and show that the properties of this
conditioned medium closely mimic those of PHA-LCM in two separate CFU-GEMM
culture systems.
Volume 64,
Issue 1,
pp. 152-155,
07/01/1984
Copyright © 1984 by The American Society of Hematology
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