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Similarities between IgG-bearing lymphocytes and hairy cells: cytologic and
cytochemical studies
T Machii and T Kitani
Six patients with hairy cell leukemia (HCL) were studied for surface
immunoglobulin ( sIg ). In all five sIg -positive cases, the heavy chain
isotype was IgG. We performed cytologic and cytochemical studies of sIgG +
lymphocytes in normal peripheral blood and compared them with hairy cells.
Normal sIgM + lymphocytes were also examined. sIgG + and sIgM + lymphocytes
made up 0.9% and 6.1% of normal peripheral blood lymphocytes, respectively.
Under a phase-contrast microscope, 76% of sIgG + lymphocytes showed
cytoplasmic processes similar to those found on hairy cells, whereas most
sIgM + lymphocytes had smooth surfaces. Tartrate-resistant acid phosphatase
(TRAP) staining revealed that TRAP- positive cells accounted for 65% of
sIgG + lymphocytes and 19% of sIgM + lymphocytes. Some (8.3%) of the sIgM +
lymphocytes expressed sIgG concomitantly. When sIgM +, sIgM +, sIgG +
lymphocytes were excluded, the percentages of cells with surface processes
and of TRAP-positive cells in the remaining sIgM +, sIgG - lymphocytes were
10% and 12%, respectively. A very small proportion (0.2%) of sIgM -, sIgG -
lymphocytes had cytoplasmic processes. These results indicate that normal
sIgG + lymphocytes are cytologically and cytochemically different from most
sIgM + lymphocytes and that the phase-contrast microscopic appearances and
TRAP activity of sIgG + lymphocytes are similar to those of HCL tumor
cells.
Volume 64,
Issue 1,
pp. 166-172,
07/01/1984
Copyright © 1984 by The American Society of Hematology
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