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L Corash, JL Costa, B Shafer, JA Donlon and D Murphy
Structurally intact platelet cohorts of differing densities can be isolated
from normal subjects by the use of isosmolar arabinogalactan density
gradients. Using platelets separated in this fashion, we have studied the
density-dependent distribution of four subcellular organelles:
mitochondria, lysosomes, dense bodies, and alpha granules. Mitochondria,
which are not secreted during platelet release, demonstrate a slow decline
in monoamine oxidase activity within the gradient. Lysosomal
beta-glucuronidase does not vary significantly with platelet density. In
contrast, dense body number and endogenous serotonin content decrease
significantly with decreasing platelet density, primarily as the result of
differences in the number of storage organelles. Platelet factor 4 content
declines rapidly in comparison to lysosomal activities (P less than .001
from bottom to top of the gradient); but beta-thromboglobulin, also an
alpha granule component, exhibits considerably less change than platelet
factor 4 (P less than .001). Thus, specific platelet subcellular
constituents have different density distributions. We postulate that these
density differences may be due to differential in vivo loss of selective
biochemical constituents from unique subcellular compartments.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
