Differential expression of HLA-DR and HLA-DC/DS molecules in a patient with
hairy cell leukemia: restoration of HLA-DC/DS expression by (12-0-
tetradecanoyl phorbol-13-acetate), 5 azacytidine, and sodium butyrate
A Faille, P Turmel and DJ Charron
Biosynthesis and molecular structure of major histocompatibility complex
(MHC) class II antigens of DR2/DR7 hairy cells were analyzed by
two-dimensional polyacrylamide-gel electrophoresis (2D-PAGE). Two anti-
human Ia monoclonal antibodies (mAb) were used to immunoprecipitate DR and
DR-linked DC/DS molecules. Monoclonal antibody VI 15 C recognizes DR
(I-E-like) molecules and CA 2.06 precipitates DR and DR-linked DC/DS
(I-A-like) molecules in DR7 allotypes. Studies were performed on a pure
population of hairy cells before and after culture with phorbol ester:
12-O-tetradecanoyl phorbol-13-acetate (TPA), 5 azacytidine (5 Aza), sodium
butyrate (NA-BU), and phytohemagglutinin (PHA-P). Before any treatment,
hairy cells expressed and synthesized DR antigens: DR alpha and beta
subunits appeared both qualitatively and quantitatively normal by 2D-PAGE
profile. In contrast, the hairy cells failed to express and synthesized any
DC/DS molecule. The lack of DC/DS molecular expression was restored after
culture in presence of TPA, sodium butyrate, and 5 azacytidine, but not
after PHA-P treatment. Differential molecular expression of MHC class II
antigens in leukemic cells provides a model to define further discrete
stages of hemopoietic differentiation and study the role of these molecules
in the cellular interactions occurring during differentiation.
Volume 64,
Issue 1,
pp. 33-37,
07/01/1984
Copyright © 1984 by The American Society of Hematology
