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ML Cleary, GS Wood, R Warnke, J Chao and J Sklar
Studies of hairy cell leukemia have yielded conflicting data about the cell
of origin in this disease. To investigate this issue, we have examined the
state of immunoglobulin genes in the cells of 11 randomly selected spleens
showing histologic involvement with hairy cell leukemia. DNA was extracted
from splenic tissue samples and digested with restriction endonucleases.
Following agarose gel electrophoresis and transfer to nitrocellulose
filters or activated nylon membranes, splenic DNA was hybridized with
radiolabeled DNA fragment probes specific for the constant regions of the
immunoglobulin heavy chain and kappa and lambda light chain genes.
Autoradiograms of the hybridized DNA in each case revealed rearrangements
of a heavy chain gene and at least one light chain gene. In addition,
immunophenotyping of cellular immunoglobulin polypeptides was carried out
on frozen tissue sections from all but one case. In each case in which an
immunoglobulin polypeptide could be detected, a rearrangement was present
in the DNA of the corresponding immunoglobulin gene. These studies offer
strong evidence for endogenous immunoglobulin synthesis in hairy cells and
for the B lymphocytic character of this leukemia.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
