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Analysis of differentiation of mouse hemopoietic stem cells in culture by
sequential replating of paired progenitors
J Suda, T Suda and M Ogawa
Blast cell colonies seen in cultures of spleen cells from 5-
fluorouracil-treated mice provide a highly enriched population of primitive
hemopoietic progenitors. Our recent studies of the differentiation
potentials of the paired daughter cells of these progenitors showed
different patterns of differentiation in the colonies produced by the
separated daughter cells. In this study, we carried out sequential
micromanipulation of paired progenitors followed by cytologic examinations
of the colonies derived from these progenitors. Of the total 94 evaluable
cultures, consisting of three or more colonies, 52 consisted of macrophage
colonies and one consisted of megakaryocyte colonies. In the remaining 41
cultures, diverse combinations of colonies revealing heterogeneous
compositions of cell lineages were identified. Presumptive genealogic trees
of the differentiation of hemopoietic progenitors constructed for the
latter group of cultures suggested that monopotent progenitors may be
derived from pluripotent progenitors in two ways: (1) directly during one
cell division of pluripotent cells or (2) as a result of progressive
lineage restriction during successive division of the pluripotent
progenitors. The results also suggested that some of the oligopotent
progenitors are capable of limited self-renewal.
Volume 64,
Issue 2,
pp. 393-399,
08/01/1984
Copyright © 1984 by The American Society of Hematology
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