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Characterization of a novel HTLV-infected cell line
HP Koeffler, IS Chen and DW Golde
A man from Chile developed an aggressive mature T cell leukemia associated
with marked eosinophilia. The neoplastic lymphocytes were of T helper
surface phenotype, and they expressed the p24 and p19 antigens of human T
cell leukemia virus (HTLV). A cell line (ME) was established from the
patient's peripheral blood cells that was initially composed of eosinophils
and T and B lymphocytes. The B lymphocytes of the cell line are polyclonal
and contain Epstein-Barr virus (EBV) DNA. Many of the T lymphocytes, a few
of the B lymphocytes, and none of the eosinophils express HTLV p19 and p24
antigens. By 6 months of culture, the ME line no longer contained
eosinophils. A variant line of ME was established; this variant (ME-2) is
notable because the cells (greater than 80%) adhere tightly to the bottom
of the culture flask; they do not express T lymphocyte markers, but 30% of
the cells contain cytoplasmic mu heavy immunoglobulin chains. These pre-B
and null lymphocytes contain p19 and p24 antigens (80% of cells), have the
HTLV- I genome, and are able to transform normal T lymphocytes in vitro. We
isolated a B lymphocyte clone (11A) from ME that expresses cytoplasmic
immunoglobulin (70% of cells) and p19 and p24 antigens (75% of cells),
contains the EBV and HTLV genomes, and can transform T lymphocytes from
normal volunteers. These data show that B lymphocytes can be infected with
HTLV, although no disease of HTLV-infected B lymphocytes has been reported.
The T lymphocytes from normal adult peripheral blood were easily
immortalized (about 70% efficiency) by cocultivation with lethally
irradiated ME cells. Twenty-five of 27 of the transformant lines were
composed of T lymphocytes with helper antigens, and two of the lines were
of T suppressor antigen phenotype. All the cell lines that were tested
constitutively produce lymphokines, including colony- stimulating factor
(CSF), erythroid-potentiating activity (EPA), macrophage
migration-inhibitory factory (MIF), neutrophil-inhibitory factor (NIF), and
differentiation-inducing factor (DIF).
Volume 64,
Issue 2,
pp. 482-490,
08/01/1984
Copyright © 1984 by The American Society of Hematology
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