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W Deimann, M Seitz, D Gemsa and HD Fahimi
The development of peroxidase (PO) reaction in the nuclear envelope (NE)
and endoplasmic reticulum (ER) of monocytes differentiating in vitro and
its relationship with arachidonic acid metabolism were studied. The PO, as
visualized by the diaminobenzidine (DAB) technique, appeared in the NE and
ER of the majority of monocytes within 24 hours of culture, with a
substantial decrease thereafter. The influence of three major groups of
agents--inhibitors of PO, of prostanoids, and of protein biosynthesis--upon
the development of the PO reaction was examined. When aminotriazole, a PO
inhibitor, was added to the culture medium, the appearance of PO was
suppressed in the monocytes. The cyclooxygenase blocker, indomethacin,
however, did not influence the development of PO. Also the blockers of
protein synthesis, puromycin, cycloheximide, and actinomycin D, did not
affect the appearance of PO. The prostanoids released from the monocytes,
ie, prostaglandin E and thromboxane B2, were determined by radioimmunoassay
and showed a time sequence of secretion that corresponded to the appearance
of PO in the cells: a marked increase within the first 24 hours with a
substantial decrease thereafter. The presence of the PO inhibitors
aminotriazole and sodium azide in the culture medium produced a suppression
of prostanoid release from the monocytes comparable with that of
indomethacin. The data suggest that the PO in the NE and ER of
differentiating monocytes in vitro (1) is associated with arachidonic acid
metabolism, and (2) is not formed by de novo protein synthesis but rather
by an activation process.
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
