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Characteristic expression of glycosphingolipid profiles in the bipotential
cell differentiation of human promyelocytic leukemia cell line HL-60
H Nojiri, F Takaku, T Tetsuka, K Motoyoshi, Y Miura and M Saito
Changes of glycosphingolipids (GSLs) in the bipotential cell
differentiation of human promyelocytic leukemia cell line HL-60 cells were
investigated by high-performance thin-layer chromatography (HPTLC), with
special reference to morphological and functional changes, such as
phagocytosis and nitroblue tetrazolium (NBT) reduction. Nine molecular
species of neutral GSLs and 13 or more species of sialo-GSLs, ie,
gangliosides, were detected on the HPTLC chromatograms for untreated HL-60
cells. The major components were ceramide dihexoside (CDH), GM3, and
sialo-paragloboside (SPG). When HL- 60 cells were induced to differentiate
into both myeloid mature cells and macrophage-like cells in vitro, no new
molecular species of GSLs specific for one of the cell differentiations was
induced, but distinctive quantitative changes in the GSL composition were
definitely observed between the two cell differentiations. During the
myeloid differentiation induced by either dimethylsulfoxide (DMSO) or
retinoic acid (RA), CDH, paragloboside (PG), and gangliosides having longer
sugar moieties characteristically increased with a concomitant decrease of
GSLs with shorter sugar chains, such as ceramide monohexoside (CMH) and
GM3, and the GSL composition profile of myeloid differentiation- induced
HL-60 cells became more similar to that of normal human granulocytes.
However, some marked differences were noted between the induced HL-60 cells
and the normal granulocytes, especially in the ganglioside compositions.
These differences might reflect either some deficiency in the in vitro
myeloid differentiation or some leukemic properties of HL-60 cells. In
marked contrast to the change of GSL composition during myeloid
differentiation, a remarkable increase of GM3, with a concurrent marked
decrease of CDH, was observed in the process of cell differentiation into
macrophage-like cells with 12-O- tetradecanoyl-phorbol-13-acetate (TPA),
which suggested an increase in the biosynthesis of GM3. These results
demonstrate that HL-60 cells express distinct GSL profiles, depending not
only on maturation stages but also on differentiation directions.
Volume 64,
Issue 2,
pp. 534-541,
08/01/1984
Copyright © 1984 by The American Society of Hematology
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