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Filterability of subpopulations of leukocytes: effect of pentoxifylline
EA Schmalzer and S Chien
Human leukocytes were separated by density into two fractions, one
containing predominantly granulocytes (FI) and the other, lymphocytes and
monocytes (FII). The filterability of these fractions and their mixture was
determined from the pressure measured during constant flow through
5-microns Nuclepore filters. The pressure-time curve of FI indicates the
behavior of a relatively homogeneous cell population. The FII pressure-time
curve can be analyzed to distinguish the effect of the more numerous and
more filterable lymphocytes from that of the sparser but less filterable
monocytes. Pressure generated by mixed leukocytes, which had been treated
with 1 and 10 mmol/L of pentoxifylline (PTX) or its metabolite I, was
substantially less than untreated control; at 10 mmol/L, the pressure was
reduced to about 50% of control. PTX appears to affect the filterability of
monocytes and polymorphonuclear leukocytes but not lymphocytes. Scanning
electron microscopy showed an inhibition of protopod formation in the
treated granulocytes. The degree of cell adhesion to the filter, as
measured by the number of cells remaining on the outflow side of the
filter, was similar in all groups (FI and FII, treated and untreated). The
results indicate that the variations in filterability between the leukocyte
subpopulations and the improvement by PTX treatment reflect differences in
the cells' ability to deform under the test conditions.
Volume 64,
Issue 2,
pp. 542-546,
08/01/1984
Copyright © 1984 by The American Society of Hematology
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