Human erythrocyte antigens: II. The In(Lu) gene regulates expression of an
antigen on an 80-kilodalton protein of human erythrocytes
MJ Telen, TJ Palker and BF Haynes
We have previously shown that a murine monoclonal antibody (A3D8)
identifies a human erythrocyte protein antigen whose expression is
regulated by the Lutheran inhibitor [In(Lu)] gene. In the present study, we
demonstrated by immunoprecipitation and Western blot techniques that the
antigen defined by A3D8 was on an 80-kD erythrocyte membrane protein. A
second 170-kD protein was coprecipitated with the 80-kD protein but failed
to show antigen activity by Western blot analysis. The 170-kD protein, when
analyzed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis in
two dimensions, was composed of 50- and 30-kD disulfide-linked subunits.
In(Lu) Lu[a-b-) erythrocytes differed from Lu(a+b+) or Lu(a-b+)
erythrocytes in that In(Lu) deoxycholate erythrocyte membrane extracts
contained trace amounts of immunoprecipitable 80-kD protein compared with
detergent-solubilized erythrocyte membrane extracts prepared from Lu(a+b+)
or Lu(a-b+) subjects.
Volume 64,
Issue 3,
pp. 599-606,
09/01/1984
Copyright © 1984 by The American Society of Hematology
