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Genetic polymorphism of platelet glycoprotein Ib
M Moroi, SM Jung and N Yoshida
Platelet glycoprotein (GP) Ib from 131 healthy Japanese was analyzed using
SDS-polyacrylamide gel electrophoresis and specific staining with
peroxidase-coupled wheat germ agglutinin after it was transferred to
nitrocellulose membranes. Four slightly different species of GPIb were
observed and designated as A, B, C, and D for glycoproteins with molecular
weights of 168,000, 162,000, 159,000, and 153,000 daltons, respectively.
The respective gene frequencies were calculated to be .073, .011, .561, and
.355 for A-, B-, C-, D-type GPIb. Portions from each type of GPIb molecule
(alpha-chain and glycocalicin) showed heterogeneity with the same molecular
weight difference, indicating that the variance would be derived from the
polypeptide portion that is exposed to the outer medium. The different
types of GPIb were the same with respect to their accessibility to
lactoperoxidase, reactivity to lectins, and affinity to TLCK-thrombin.
Although Bolin et al reported patients with a bleeding tendency whose
platelets have double GPIb bands, here we found that platelets with
different GPIb phenotypes showed no significant differences in aggregating
activity and platelet retention. Analysis of GPIb phenotype should be
important for structural and physiologic studies on GPIb and glycocalicin.
Volume 64,
Issue 3,
pp. 622-629,
09/01/1984
Copyright © 1984 by The American Society of Hematology
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