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RL Edwards and D Perla
Human monocytes generate the procoagulant tissue factor (MTF) following
exposure to a variety of immune stimuli in vitro. The generation of MTF is
modified by T cells, lymphokines, and immunoregulatory lipoproteins, and
recent studies have shown that MTF can be activated in an immune- specific
manner following exposure to antigen. We have examined the role of serum
factors in the regulation of MTF generation. Low concentrations (less than
1%) of heat-inactivated normal human serum greatly enhanced MTF generation
in cultures of normal peripheral blood mononuclear cells. The stimulatory
effect was observed in cultures of both unstimulated cells and cells
exposed to bacterial lipopolysaccharide. Stimulation was not observed at
high serum concentrations (greater than 10%) and could not be explained by
endotoxin contamination or activation of the assay system. Stimulatory
activity was present in plasma and BaSO4-adsorbed plasma as well as
autologous and allogeneic serum, was not abolished by removal of serum
lipoproteins, and did not require the presence of T cells for its
expression. Sera from 28 different normal volunteers were screened for
stimulatory activity and demonstrated a wide variation in potency. These
results suggest that a potent factor is present in sera that enhances the
expression of MTF activity in vitro. This factor is distinct from
previously described lipoprotein regulators and may play a role in the
initiation of coagulation in both normal hemostasis and pathologic states.
This article has been cited by other articles:
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| Copyright © 1984 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
