Hemin-induced conversion of pyruvate kinase isozymes in K562 cells
S Takegawa, T Shinohara and S Miwa
The effects of hemin on the conversion of pyruvate kinase (PK) isozymes
from the M2-type to the L-type in K562 cells were investigated.
Immunofluorescence, ion exchange chromatography, and electrophoretic
studies showed that the untreated K562 cells contained only the M2-type PK,
while eight to 20 days after induction with hemin, concomitant with
hemoglobin F synthesis, L-type PK levels increased while M2-type PK levels
decreased. Electrophoretic study revealed three hybrid isozymes of the
L-type and M2-type PK. We conclude that the conversion of PK isozymes from
the M2-type to the L-type in erythroid precursor cells occurs in the early
stage of maturation.
Volume 64,
Issue 3,
pp. 754-757,
09/01/1984
Copyright © 1984 by The American Society of Hematology
