Different colony-stimulating factors are detected by the "interleukin-
3"-dependent cell lines FDC-Pl and 32D cl-23
AJ Hapel, HS Warren and DA Hume
The cell lines FDC-Pl and 32D cl-23 have previously been used as unique
indicators for the growth-promoting activity of interleukin-3. We show that
FDC-Pl cells respond to granulocyte/macrophage colony-stimulating factor
(GM-CSF, CSF-2) as well as to interleukin-3. In keeping with this finding,
FDC-Pl cells express the macrophage-specific marker, F4/80. FDC-Pl cells do
not, however, respond to macrophage CSF (M-CSF, CSF-1). In contrast, 32D
cl-23 cells do not respond to GM-CSF and lack F4/80. Instead, 32D cl-23
cells respond to an as yet undefined factor in conditioned medium (CM) from
the primate T cell line, MLA-144, and CM from mitogen-stimulated human
lymphocytes (HLCM). 32D cl-23 cells are Lyt-1+. Both FDC-Pl and 32D cl-23
cells consume interleukin-3, but only FDC Pl cells consume GM-CSF.
Similarly, 32D cl-23, but not FDC-Pl, cells consume 32D cl-23 growth factor
from MLA-144 CM and HLCM. Interleukin-3-dependent cell lines must therefore
concurrently express different functional cell surface receptors for a
variety of biochemically distinct growth factors.
Volume 64,
Issue 4,
pp. 786-790,
10/01/1984
Copyright © 1984 by The American Society of Hematology
