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Differentiation stimuli induce receptors for plasma fibronectin on the
human myelomonocytic cell line HL-60
CG Pommier, J O'Shea, T Chused, T Takahashi, M Ochoa, TB Nutman, C Bianco and EJ Brown
Plasma fibronectin (Fn) induces phagocytosis of C3b-opsonized sheep
erythrocytes (EC3b) by human peripheral blood monocytes. However, Fn does
not induce erythrophagocytosis of EC3b by human polymorphonuclear
leukocytes (PMN), unless the PMN have been exposed to C5a or N-formyl-
methionyl-leucyl-phenylalanine. Because of this difference, it is of great
interest to examine Fn binding to cells that possess the capacity to
differentiate into either granulocytes or monocytes. Hence, we have
examined the consequences of Fn binding to the human myelomonocytic cell
line, HL-60, both before and after in vitro differentiation of the HL-60,
along a monocytoid or a granulocytoid pathway. Fn receptors were not found
on undifferentiated HL-60, but several differentiating agents promoted the
HL-60 binding of Fn-coated microspheres (Fn-ms). The peak of Fn-ms binding
occurred four to five days after the induction of differentiation with
dimethylsulfoxide (DMSO), and two days after induction by PMA. In addition,
cells that differentiated along either the monocytoid or the granulocytoid
pathway showed a marked increase in the phagocytosis of both IgG-coated
erythrocytes (EA) and EC3b when they were exposed to Fn. Comparison of the
effects of anti-Fn monoclonals on the binding of Fn-ms to the monocytes,
PMN, and HL-60 showed that the same monoclonals block Fn-ms-binding and
Fn-induced EC3b phagocytosis by all three cell types. Two monoclonal
antibodies, M1/70 and A6F10, directed against membrane antigens on PMN and
monocytes, inhibited Fn-ms binding. Both also blocked Fn-induced EC3b
ingestion by these cells. However, neither antibody blocked Fn-ms binding
or EC3b ingestion by differentiated HL-60. We conclude that differentiated
HL-60 cells express functionally active Fn receptors, similar to monocytes
and activated PMN, which, nonetheless, differ from normal cells in their
association with the antigens recognized by M1/70 and A6F10.
Volume 64,
Issue 4,
pp. 858-866,
10/01/1984
Copyright © 1984 by The American Society of Hematology
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