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Differentiation of a human monocytic cell line by 1,25-dihydroxyvitamin D3
(calcitriol): a morphologic, phenotypic, and functional analysis
WF Rigby, L Shen, ED Ball, PM Guyre and MW Fanger
Several recent studies have suggested a role for 1,25-dihydroxyvitamin D3
(calcitriol) in myeloid differentiation. We have examined the effects of
calcitriol on the U937 monoblast cell line and found that calcitriol, at
near physiologic concentrations, is a potent inhibitor of U937 growth.
Moreover, calcitriol induces differentiation to a monocyte-macrophage
phenotype marked by enhanced alpha-naphthyl esterase staining. Morphologic
changes were attended by de novo induction of the myeloid specific antigen
detected by the monoclonal antibody AML-2-23, as well as dramatic increases
in Fc receptors for IgG. In addition, calcitriol induced U937 cells to
perform phagocytosis and antibody-dependent cellular cytotoxicity. These
results indicate the potential activity of calcitriol in myeloid
differentiation and additionally suggest a role for calcitriol in
monocyte-macrophage activation.
Volume 64,
Issue 5,
pp. 1110-1115,
11/01/1984
Copyright © 1984 by The American Society of Hematology
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