A method for simultaneous study of the karyotype, morphology, and
immunologic phenotype of mitotic cells in hematologic malignancies
L Teerenhovi, S Knuutila, M Ekblom, L Rossi, GH Borgstrom, JK Tallman, L Andersson and A de la Chapelle
A major problem in the cytogenetic analysis of hematologic neoplasms has
been an inability to identify the cell from which the chromosomes were
obtained. We describe a procedure that allows simultaneous analysis of
karyotype and cell cytology in mitotic cells. The method differs from
conventional cytogenetic analysis in that after mild hypotonic treatment,
the cells are cytocentrifuged onto glass slides. In mitotic cells, this
procedure often results in adequate spread of the chromosomes within the
intact cell membrane. The cytoplasmic structure also remains intact, so
that cytologic preparations are of good quality. Morphologic and
immunologic identification of mitotic cells can be done using routine
hematologic stains, such as Giemsa or Sudan black B, and various antisera
using immunofluorescence techniques. The chromosomes can be simultaneously
analyzed either without banding on slides stained with Giemsa or with
Q-banding on slides stained with immunofluorescence techniques.
Identification of numerical and structural karyotype aberrations thus is
possible in morphologically identified cells.
Volume 64,
Issue 5,
pp. 1116-1122,
11/01/1984
Copyright © 1984 by The American Society of Hematology
