Lipid analyses and fluidity studies by electron spin resonance of red cell
membranes in hereditary high red cell membrane phosphatidylcholine
hemolytic anemia
Y Yawata, T Sugihara, M Mori, S Nakashima and Y Nozawa
Membrane lipid analyses and electron spin resonance (ESR) studies of
membrane fluidity were carried out on the red cells of a Japanese patient
with hereditary high red cell membrane phosphatidylcholine hemolytic anemia
(HPCHA). Increased amounts of phosphatidylcholine (PC) and cholesterol were
found in the membrane lipids of the affected patient, despite normal plasma
lipids. The order parameter of cholesterol-free pure phospholipid liposomes
prepared from this patient's red cells was decreased, apparently because of
the increased PC. In contrast, the order parameter of the total red cell
lipid liposomes (containing free cholesterol) was essentially normal. The
overall fluidity of the intact red cells was determined by ESR with a spin
probe, 5-SAL. Again, the order parameters were normal in the intact red
cells of the patient with HPCHA. This suggests that the concomitant
increase of membrane cholesterol and phosphatidylcholine serves to maintain
normal membrane fluidity in the HPCHA red cells.
Volume 64,
Issue 5,
pp. 1129-1134,
11/01/1984
Copyright © 1984 by The American Society of Hematology
