SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Keating, A. Articles by Singer, J. W. Search for Related Content PubMed PubMed Citation Articles by Keating, A. Articles by Singer, J. W. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  The generation of human long-term marrow cultures from marrow depleted of Ia (HLA-DR) positive cells A Keating, J Powell, M Takahashi and JW Singer Successful long-term cultures were generated from marrow buffy-coat cells incubated with either of two monoclonal anti-Ia antibodies, 7.2 and HBIOa, and complement. The mean CFU-GM toxicity for 7.2 and HBIOa was 81% and 94%, respectively, of the complement control. Cultures generated from marrows treated with 7.2 and complement produced between 30% and 163% of the CFU-GM (mean, 78.2 +/- 52.4%) and 33% of the BFU-E produced by the complement control cultures. Long-term cultures from marrows treated with the more cytotoxic HBIOa antibody yielded 261% of the CFU-GM present at culture initiation. Our data suggest that the progenitor cells necessary for generation of long-term cultures from human marrow are Ia-negative and may represent less mature stem cells than those measurable by semisolid colony assays. Furthermore, marrow treated with cytotoxic anti-Ia antibodies may be suitable for use in autologous transplantation. Volume 64, Issue 6, pp. 1159-1162, 12/01/1984 Copyright © 1984 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Eridani Stem cells for all seasons? Experimental and clinical issues J R Soc Med, January 1, 2002; 95(1): 5 - 8. [Full Text] [PDF] D. J. Prockop Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues Science, April 4, 1997; 276(5309): 71 - 74. [Abstract] [Full Text] M. O. Muench, M. G. Roncarolo, and R. Namikawa Phenotypic and Functional Evidence for the Expression of CD4 by Hematopoietic Stem Cells Isolated From Human Fetal Liver Blood, February 15, 1997; 89(4): 1364 - 1375. [Abstract] [Full Text] [PDF] K. Inoue, H. Ogawa, Y. Sonoda, T. Kimura, H. Sakabe, Y. Oka, S. Miyake, H. Tamaki, Y. Oji, T. Yamagami, et al. Aberrant Overexpression of the Wilms Tumor Gene (WT1) in Human Leukemia Blood, February 15, 1997; 89(4): 1405 - 1412. [Abstract] [Full Text] [PDF]

	Copyright © 1984 by American Society of Hematology         Online ISSN: 1528-0020