Stimulation of prostacyclin synthesis in vascular cells by mononuclear cell
products
E Dejana, F Breviario, G Balconi, V Rossi, G Remuzzi, G de Gaetano and A Mantovani
Supernatants were obtained from human peripheral blood mononuclear cells
stimulated with phytohemagglutinin or in a mixed lymphocyte reaction. The
effect of mononuclear cell products on vascular prostacyclin (PGI2)
production was measured using cultured rat aortic smooth muscle cells (SMC)
or aortic rings. PGI2 was measured by radioimmunoassay of its metabolite,
6-keto-PGF1 alpha. Supernatants containing mononuclear cell products
induced PGI2 production in vascular tissue. Supernatant-induced PGI2
production of SMC was relatively slow, requiring more than six hours of
incubation with supernatants, and was completely prevented by aspirin, a
cyclooxygenase inhibitor. The regulation of arachidonic acid metabolism by
products of stimulated mononuclear cells, which is critical to the
physiology and pathology of blood vessels, may be an important aspect of
the interaction between immunocompetent cells and vascular tissue.
Volume 64,
Issue 6,
pp. 1280-1283,
12/01/1984
Copyright © 1984 by The American Society of Hematology
