Monoclonal antibody AHN-1 inhibits phagocytosis by human neutrophils
KM Skubitz, DJ Weisdorf and PK Peterson
The granulocyte-specific monoclonal antibody, AHN-1, immunoprecipitates two
major surface-iodinated proteins of 105,000 and 145,000 to 150,000 daltons
from normal human neutrophils. In this study, the effect of AHN- 1 on a
number of neutrophil functions was evaluated in vitro. Both complement- and
antibody-mediated phagocytosis were inhibited when human neutrophils were
pretreated with AHN-1 and opsonized bacteria were used as targets. The
inhibition of phagocytosis was specific, in that lysosomal enzyme release
and chemotaxis were not altered by treatment with AHN-1. AHN-1 did inhibit
superoxide production by neutrophils in response to particulate stimuli,
but not in response to the soluble stimulus,
12-O-tetradecanoylphorbol-13-acetate. The data indicate that one or both of
these surface proteins may be important in the process of phagocytosis.
AHN-1 should be useful in isolating and further characterizing the nature
of these molecules.
Volume 65,
Issue 2,
pp. 333-339,
02/01/1985
Copyright © 1985 by The American Society of Hematology
