|
|
 Previous Article | Table of Contents | Next Article 
A monoclonal anti-human platelet antibody: a new platelet aggregating
substance
M Higashihara, H Maeda, Y Shibata, S Kume and T Ohashi
A monoclonal anti-human platelet antibody, TP82, is described, which caused
irreversible aggregation of platelets in association with the release of
adenosine triphosphate or [14C] serotonin, and which inhibited
ristocetin-induced agglutination. Immunofluorescence assay showed that the
antibody binds to platelets, megakaryocytes, and common acute lymphoblastic
leukemia cells. The antibody (IgG1) immunoprecipitated a polypeptide of
23,000 daltons with an isoelectric point of about 7.0. The aggregation
induced by the purified antibody and/or F(ab')2 fragments occurred in
platelet-rich plasma and with washed platelets, but not with formalin-fixed
washed platelets. TP82- induced aggregation was completely inhibited by
disodium ethylendiaminotetraacetate, diltiazem, W-7, PGE1, and several
metabolic inhibitors. At a concentration of apyrase or CP/CPK, which
inhibited adenosine 5-diphosphate-induced aggregation. TP82-induced
aggregation was only partially affected. Thrombin was not required for the
antibody- mediated effects, since two thrombin inhibitors failed to block
the reaction. The antibody, at least at a high concentration, induced
platelet aggregation by a mechanism almost independent of thromboxane A2
formation, since cyclooxygenase inhibitors had little inhibitory effect on
aggregation. TP82 monoclonal antibody is a new platelet- aggregating
substance that interacts with a low-molecular-weight binding site on the
platelet membrane.
Volume 65,
Issue 2,
pp. 382-391,
02/01/1985
Copyright © 1985 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Y. Murayama, J.-i. Miyagawa, K. Oritani, H. Yoshida, K. Yamamoto, O. Kishida, T. Miyazaki, S. Tsutsui, T. Kiyohara, Y. Miyazaki, et al.
CD9-mediated activation of the p46 Shc isoform leads to apoptosis in cancer cells
J. Cell Sci.,
July 1, 2004;
117(15):
3379 - 3388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Park, T. Inoue, M. Ueda, T. Hirano, T. Higuchi, I. Konishi, H. Fujiwara, and S. Fujii
Anti-CD9 monoclonal antibody-stimulated invasion of endometrial cancer cell lines in vitro: possible inhibitory effect of CD9 in endometrial cancer invasion
Mol. Hum. Reprod.,
August 1, 2000;
6(8):
719 - 725.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hirano, T. Higuchi, M. Ueda, T. Inoue, N. Kataoka, M. Maeda, H. Fujiwara, and S. Fujii
CD9 is expressed in extravillous trophoblasts in association with integrin {alpha}3 and integrin {alpha}5
Mol. Hum. Reprod.,
February 1, 1999;
5(2):
162 - 167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hirano, T. Higuchi, H. Katsuragawa, T. Inoue, N. Kataoka, K. R. Park, M. Ueda, M. Maeda, H. Fujiwara, and S. Fujii
CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line, BeWo cells
Mol. Hum. Reprod.,
February 1, 1999;
5(2):
168 - 174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Deckmyn, K. Vanhoorelbeke, N. Cauwenberghs;, S. Nomura, and Y. Ozaki
A Platelet-Activating Antiglycoprotein Ib Monoclonal Antibody
Blood,
November 1, 1997;
90(9):
3807 - 3808.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Banerjee, M. Hadjiargyrou, and P. H. Patterson
An Antibody to the Tetraspan Membrane Protein CD9 Promotes Neurite Formation in a Partially alpha 3beta 1 Integrin-Dependent Manner
J. Neurosci.,
April 15, 1997;
17(8):
2756 - 2765.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ozaki, K. Satoh, K. Kuroda, R. Qi, Y. Yatomi, S. Yanagi, K. Sada, H. Yamamura, M. Yanabu, S. Nomura, et al.
Anti-CD9 Monoclonal Antibody Activates p72[IMAGE] in Human Platelets
J. Biol. Chem.,
June 23, 1995;
270(25):
15119 - 15124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
