Bone marrow from cadaver donors for transplantation
H Mugishima, P Terasaki and A Sueyoshi
To determine the feasibility of obtaining bone marrow cells from cadaver
donors for transplantation, marrow cells were prepared from 17 cadaver
donors. After surgical removal of the iliac crest, as many as 2 X 10(9)
cells were isolated. Cadaver marrow had a lower percentage of T cells (mean
of 10%) than did marrow from living donors. The T cells were lysed by a
monoclonal antibody and human complement to a point at which no sheep red
blood cell-rosetting cells were detected. Low levels of T colonies,
however, grew out from the monoclonal antibody-treated cells. Although cell
loss inevitably occurs from purification, antibody treatment, freezing, and
thawing, sufficient numbers can be recovered for transplantation. The yield
of stem cells was 84% for CFU-C, 39% for CFU-E, 81% for BFU-E, and 48% for
CFU-GEMM. We suggest that T cell- depleted marrow cells from cadaver donors
could be used for transplantation. Improved immunosuppressive therapy may
be required, however, to prevent graft rejection of allogeneic marrow that
may have minor histocompatibility differences.
Volume 65,
Issue 2,
pp. 392-396,
02/01/1985
Copyright © 1985 by The American Society of Hematology
