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Fibronectin in artery subendothelium is important for platelet adhesion
WP Houdijk and JJ Sixma
The role of subendothelial fibronectin in platelet interaction with
subendothelium was studied. Human umbilical artery subendothelium was
exposed to flowing blood containing 111In-labeled platelets in an annular
perfusion chamber. Platelet adhesion was determined from the 111In
radioactivity on the vessel wall. When perfusions were performed for five
minutes at a wall shear rate of 1,800 s-1, platelet adhesion was the same
whether normal plasma or fibronectin-free plasma was used. Preincubation of
subendothelium with rabbit anti-human fibronectin serum, however, resulted
in a marked inhibition of platelet adhesion. Preincubation with normal
rabbit serum had no effect. Platelet adhesion was also diminished when the
vessel wall was preincubated with anti- fibronectin IgG fraction or F(ab')2
fragment. After the latter preincubations, frozen sections of 4 micron were
incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG,
F(ab')2 fragment specific. Fluorescence was seen throughout the
subendothelium both before and after perfusion. No fluorescence was seen
when subendothelium was preincubated with normal rabbit IgG or F(ab')2 or
with anti-fibronectin IgG that had been absorbed with purified fibronectin.
After absorption of anti-fibronectin IgG with purified fibronectin, the
inhibiting effect on platelet adhesion was also no longer present.
Preincubation of the vessel wall with anti-fibronectin IgG reduced platelet
adhesion significantly at a wall shear rate of 800 s-1. This effect was
even greater at 1,800 s-1. At low shear rate (400 s-1), there was no
inhibition.
Volume 65,
Issue 3,
pp. 598-604,
03/01/1985
Copyright © 1985 by The American Society of Hematology
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