|
|
 Previous Article | Table of Contents | Next Article 
The origin of ABH antigens on human platelets
RA Dunstan, MB Simpson, RW Knowles and WF Rosse
ABH antigens are present on platelets from individuals of the corresponding
red cell phenotype, but the extent to which these antigens are intrinsic or
adsorbed remains undefined. To evaluate platelets for intrinsic H
substance, an IgM mouse monoclonal antibody against type 2H chain (the
intrinsic H structure found on erythrocytes) was labeled with 125I and
incubated with platelets from donors of different ABO type. The antibody
showed dose-response saturation curves, and binding to platelets paralleled
that of the red cell ABO type, with O greater than B greater than A1
greater than A1B greater Oh cells, giving a single factor variance F of 190
(P less than .0005). Passive adsorption of A antigens by platelets has been
previously reported. To verify this phenomenon for A and B antigens and to
quantitate the elution of A and B antigens from platelets, the following
assay system was used. Platelets from group A1 and B donors were incubated
in plasma from group O donors, and platelets from group O donors were
incubated in plasma from different ABO, Lewis, and presumed secretor-type
donors. Human IgG anti-A or anti-B was added to the platelets. The amount
of antibody bound was determined with a 125I- labeled mouse monoclonal
anti-human IgG. When incubated for 96 hours in group O plasma, group A1
platelets showed a 45% to 50% decrease in binding of anti-A. There was no
significant change in the level of type 2H antigen on these platelets
during the same incubation period. Group O platelets incubated in A or B
plasmas rapidly acquired the antigens, but if returned to their original
plasma, 95% of this passively adsorbed antigen eluted off within 18 hours.
The maximum uptake of A and B substances was influenced by the Lewis and
secretor type of donor plasma. Our present study demonstrates that ABH
antigens on platelets consist of type 2H chains, which are presumably
intrinsic as when found on red cells, and of passively adsorbed ABH
structures, which are presumably type 1H chains.
Volume 65,
Issue 3,
pp. 615-619,
03/01/1985
Copyright © 1985 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. L. W. Cooling, K. Kelly, J. Barton, D. Hwang, T. A. W. Koerner, and J. D. Olson
Determinants of ABH expression on human blood platelets
Blood,
April 15, 2005;
105(8):
3356 - 3364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. R. Curtis, J. T. Edwards, M. J. Hessner, J. P. Klein, and R. H. Aster
Blood group A and B antigens are strongly expressed on platelets of some individuals
Blood,
August 15, 2000;
96(4):
1574 - 1581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsui, T. Shimoyama, M. Matsumoto, Y. Fujimura, Y. Takemoto, M. Sako, J. Hamako, and K. Titani
ABO Blood Group Antigens on Human Plasma von Willebrand Factor After ABO-Mismatched Bone Marrow Transplantation
Blood,
October 15, 1999;
94(8):
2895 - 2900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
