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Next Article 
Platelets interact with fibrin only after activation
RR Hantgan, RG Taylor and JC Lewis
Interactions between platelets and fibrin have been visualized by phase
contrast, epifluorescence, and scanning electron microscope examination of
clots formed with dansylcadaverine-labeled fibrin and gel-filtered
platelets. After thrombin activation, the platelets appeared as fluorescent
aggregates with bridging strands of fibrin; formaldehyde- fixed platelets
were not fluorescent under the same experimental conditions. Scanning
electron micrographs demonstrated that thrombin- activated cells had
numerous pseudopods to which the fibrin strands adhered; fixed platelets
exhibited a smooth discoid appearance and did not interact with the clot.
Platelets trapped in clots formed with Batroxobin (Pentapharm) (platelets
are not activated by Batroxobin as confirmed by light-scattering
aggregometry measurements) remained as nonfluorescent, discoid cells,
whereas platelets first activated by adenosine diphosphate formed brightly
fluorescent aggregates. Light- scattering data of thrombin activation (0.2
U/mL) indicated that preincubation of platelets with 0.1 mmol/L
prostaglandin E1 (PGE1) prior to addition of thrombin decreased the extent
and rate of platelet shape change and resulted in 100-fold slower
aggregation. Clots formed in the presence of PGE1 revealed decreased
fluorescence intensity and fewer platelet-fibrin contacts. Gly-Pro-Arg-Pro,
which blocks fibrinogen binding and fibrin assembly, was also effective in
blocking platelet-fibrin interactions. These results indicate that platelet
activation is a prerequisite for attachment of platelets to fibrin.
Volume 65,
Issue 6,
pp. 1299-1311,
06/01/1985
Copyright © 1985 by The American Society of Hematology
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