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AJ Hapel, MC Fung, RM Johnson, IG Young, G Johnson and D Metcalf
Interleukin-3 (IL-3) (multipotential colony-stimulating factor [multi- CSF]
) is an important regulator of hemopoiesis. We recently isolated a cDNA
clone of this gene and describe in this manuscript the biologic properties
of the expressed gene product. An SV40 expression vector carrying cDNA
encoding murine interleukin-3 was constructed so that expression of the
IL-3 gene was placed under the control of the SV40 early promoter. When the
expression vector was transfected to COS-1 monkey cells, IL-3 activity was
secreted into the medium, reaching maximal levels 72 hours after
transfection. The IL-3 produced by the COS-1 cells was partially purified
using diethylaminoethyl Sephacel and phenyl-Sepharose, and its
chromatographic properties were the same as IL-3 produced by the WEHI-3
cell line. The biologic activities of the "expressed" IL-3 include the
"induction" of 20-alpha-hydroxysteroid dehydrogenase (20-alpha-SDH) in
splenic lymphocytes from nu/nu mice, proliferative activity for 32D cl-23
and FDC-P1 cell lines, and colony- stimulating activity for
granulocyte-macrophage, eosinophil, megakaryocyte, natural killer-like,
erythroid, and multipotential colony-forming cells from murine fetal liver
and adult bone marrow.
This article has been cited by other articles:
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| Copyright © 1985 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
