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Neutrophils adherent to a nonphagocytosable surface (glomerular basement
membrane) produce oxidants only at the site of attachment
MC Vissers, WA Day and CC Winterbourn
Adherence of neutrophils to glomerular basement membrane containing
immunoglobulin G aggregates was accompanied by a marked increase in oxygen
uptake (eightfold). Very little of the O2 consumed was recovered as
superoxide, measured by cytochrome c reduction, or as H2O2, measured with
horseradish peroxidase and scopoletin. When neutrophils were incubated with
the basement membrane preparation in the presence of cerium chloride to
detect H2O2, electron micrographs showed cerium perhydroxide deposits in
the contact area between the cells and the basement membrane, but not on
the remainder of the cell surface. The results imply that superoxide is
produced only where the plasma membrane is in contact with the basement
membrane matrix, and that it mostly breaks down to H2O2 or undergoes other
reactions at this site. The longer lifetime of H2O2 compared with that of
superoxide allows some of the H2O2 produced to be detected in the medium.
The results also suggest that the area of contact between the neutrophil
and surfaces such as basement membrane is inaccessible to proteins in the
medium, eg, cytochrome c. Circulating scavengers such as superoxide
dismutase or catalase, or proteolytic inhibitors, may therefore be unable
to control events occurring at this site.
Volume 66,
Issue 1,
pp. 161-166,
07/01/1985
Copyright © 1985 by The American Society of Hematology
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