Hematopoietic factor production by a cell line (TC-1) derived from adherent
murine marrow cells
ZX Song, RK Shadduck, DJ Innes , A Waheed and PJ Quesenberry
An adherent cell line, termed TC-1, has been isolated from long-term liquid
culture of murine marrow cells by repeated exposure of the adherent cells
to 0.1% trypsin. This is an alkaline phosphatase- positive cell line
showing variable staining with acid phosphatase and alpha-naphthyl acetate
esterase. On electron microscopy, the cells have moderate amounts of rough
endoplasmic reticulum and variable numbers of polyribosomes. Some cells
contain large clusters of laked glycogen particles. Intermediate junctions
are present between some cells. Conditioned medium from this cell line
produced from 384 to 638 units of CSF-1 per milliliter by radioimmunoassay
and a CSF-1-dependent synergistic activity, which stimulates giant
macrophage colony formation of marrow cells in soft agar. The conditioned
medium also stimulates 3H-TdR incorporation by marrow cells in liquid
culture and induces secondary adherent cell lines. The growth factor(s)
produced by the TC-1 stromal cell line may be important in the regulation
of early stages of hematopoietic differentiation. Two subclones, TC-1-C-11
and TC-1-C-3, have been isolated from passage 25 of the TC-1 cells by a
penicylinder separation technique. The TC-1-C-11 is phenotypically like the
parent TC-1 line and produces macrophage growth factors. The TC-1-C- 3
grows as an epithelioid monolayer with visible junctions among adjacent
cells under phase contrast microscopy. This subclone produces retrovirus
and is capable of providing anchorage support for hematopoietic stem cells.
The TC-1 cell line and its subclones may provide models for the control of
early stem cell proliferation and differentiation.
Volume 66,
Issue 2,
pp. 273-281,
08/01/1985
Copyright © 1985 by The American Society of Hematology
