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Heterogeneity of human peripheral blood eosinophil-type colonies: evidence
for a common basophil-eosinophil progenitor
JA Denburg, S Telizyn, H Messner, B Lim, N Jamal, SJ Ackerman, GJ Gleich and J Bienenstock
We have recently shown that a proportion of previously designated human
eosinophil "(Eo)-type" colonies in methylcellulose contain basophils and
histamine (Denburg et al Blood 61:775, 1983). In the present studies,
individual Eo-type colonies have been analyzed by cell morphology as well
as by biochemical assays for histamine, Charcot- Leyden crystal protein
(CLC), and eosinophil granule major basic protein (MBP). Clonal origin of
single Eo-type colonies was confirmed by G6PD isoenzyme analysis.
Morphological observations of such colonies revealed the existence of two
distinct colony types: (1) Eo type containing 100% basophils and (2) Eo
type containing mixtures of basophils and eosinophils, including cells with
mixed basophil- eosinophil granulation. Histamine was not detected in pure,
mature peripheral blood eosinophils. Immunofluorescent studies demonstrated
bright staining for CLC and MBP in 95% +/- 3% of cells in Eo-type colonies
but only in 5% +/- 4% of cells in GM-type colonies. Radioimmunoassay for
MBP was positive in 5/9 Eo-type and 0/10 neutrophil-macrophage ("GM-type")
colonies, with a mean level (nanogram/colony) of 11.6 +/- 4.2 per Eo-type
colony; four of the latter colonies were doubly positive for both histamine
and MBP. These and previous findings point out the morphological and
biochemical heterogeneity of peripheral blood Eo-type colonies and provide
direct evidence for the existence of a common, circulating
basophil-eosinophil progenitor.
Volume 66,
Issue 2,
pp. 312-318,
08/01/1985
Copyright © 1985 by The American Society of Hematology
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