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Disparate differentiation in hemopoietic colonies derived from human paired
progenitors
AG Leary, LC Strauss, CI Civin and M Ogawa
We analyzed the differentiation of hemopoietic colonies derived from human
paired daughter cells. Candidate progenitor cells were isolated by use of a
micromanipulation technique from cultures of My-10 antigen- positive cord
blood cells. Then nine to 36 hours later, the paired daughter cells were
separated with a micromanipulator and allowed to form colonies in
methylcellulose medium containing erythropoietin, phytohemagglutinin
leukocyte-conditioned medium, and platelet-poor plasma. The cellular
composition of the colonies was determined by differentiating all of the
cells of the May-Grunwald-Giemsa-stained preparation. Of a total of 75
evaluable pairs of colonies, 35 consisted of 28 types of disparate pairs
revealing nonhomologous lineage combinations. Forty pairs were homologous
in lineage expression. However, the proportions of the individual cell
lineages were significantly different in the members of some of the
homologous pairs. Some pairs revealed significant differences in colony
size. These observations are similar to those reported for murine paired
progenitors and are consistent with the stochastic model of human stem cell
differentiation.
Volume 66,
Issue 2,
pp. 327-332,
08/01/1985
Copyright © 1985 by The American Society of Hematology
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