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JL Gabrilove, K Welte, L Lu, H Castro-Malaspina and MA Moore
Conditioned medium (CM) obtained from a human hepatoma cell line, SK-
HEP-1, contains colony-stimulating factors (CSFs) active on murine and
human bone marrow-derived granulocyte and macrophage colony-forming units
(CFU-GM) and a factor capable of inducing granulocyte-macrophage
differentiation (GM-DF) of murine myelomonocytic leukemic cells WEHI-
3B(D+) and human promyelocytic leukemic cells HL-60 when assayed in
semisolid agar cultures. The human active granulocyte-macrophage colony-
stimulating factor (GM-CSF) for day 7 CFU-GM and the GM-DF for WEHI- 3B(D+)
and for HL-60 are not separable by acrylamide agarose column
chromatography, eluting at an apparent molecular weight between 20,000 and
35,000 daltons, or by isoelectric focusing (isoelectric point, pH 5.4). In
addition, SK-HEP-1 CM contains erythroid burst-promoting activity (BPA) and
a factor that promotes the growth of human mixed colonies. SK-HEP-1 cells,
which grow as an adherent monolayer, appear not to be endothelial or
monocytic in origin since by immunofluorescent staining they are negative
for Ia (HLA-DR), monocyte antigen 1 and 2, lysozyme, and factor
VIII-related antigen. Positive immunofluorescent staining for keratin and
fibronectin suggests the possibility that SK- HEP-1 is an epithelial cell
line. Constitutive production of GM-DF as well as other hematopoietic
activities including GM-CSF, erythroid BPA, and an activity that promotes
the growth of human mixed colony progenitors by a human epithelial tumor
cell line, SK-HEP-1, suggests that this cell line is a valuable resource
for both large-scale production of these factors and the cloning of the
gene(s) that code for these regulators.
This article has been cited by other articles:
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| Copyright © 1985 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
