Surface phenotypes of human hemopoietic progenitor cells defined by
monoclonal antibodies
D Ferrero, M Gabbianelli, C Peschle, B Lange and G Rovera
A panel of ten monoclonal antibodies which react with antigens present on
the surface of myeloid leukemic cells was used to investigate the
distribution of these antigens on normal hemopoietic stem cells and
progenitor cells at various stages of maturity. A population of immature
cells, possibly stem cells, that are capable of regenerating CFU-GM in
long-term marrow cultures reacts with four antibodies recognizing antigens
abundantly expressed in leukemic cells, but does not react with antibodies
against Ia-like molecules or against carbohydrate determinants specific for
myeloid cells. Progenitor cells that form mixed colonies in semisolid
medium (CFU-GEMM), early erythroid (BFU-E) and early myelomonocytic (type 1
CFU-GM) progenitors retain the antigens present on the hypothetical stem
cell population and begin to express Ia-like antigens. As they
differentiate, myeloid and erythroid progenitors undergo a series of
quantitative and qualitative shifts in surface phenotype. They begin to
express stage- related, lineage-specific antigens and cease expressing
antigens common to early cells of different lineages. The identification of
antigens present on very immature normal progenitor cells should be
valuable in future studies aimed at the detailed characterization of this
relatively little-known hemopoietic cell population.
Volume 66,
Issue 3,
pp. 496-502,
09/01/1985
Copyright © 1985 by The American Society of Hematology
