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Common and pre-B acute lymphoblastic leukemia cells express interleukin 2
receptors, and interleukin 2 stimulates in vitro colony formation
I Touw, R Delwel, R Bolhuis, G van Zanen and B Lowenberg
The role of interleukin 2 (IL 2) as a possible regulator of in vitro
proliferation and differentiation of non-T acute lymphoblastic leukemia
(ALL) cells was investigated. For this purpose, leukemic cells from the
blood or bone marrow of eight untreated patients with common or pre-B ALL
were analyzed using the anti-Tac monoclonal antibody (reactive with the IL
2 receptor) in indirect immunofluorescence. The receptors for IL 2, which
were initially absent from the cell surface, were induced on high
percentages of the ALL cells after the in vitro exposure to the lectin
phytohemagglutinin or the phorbol ester 12-O-
tetradecanoylphorbol-13-acetate in six patients, suggesting that the cells
had become sensitive to IL 2. In colony cultures to which feeder leukocytes
and IL 2 had been added, colony growth was obtained in five of eight cases.
Whereas the cells from one patient formed colonies in the absence of
exogenous stimuli, the cells from others were dependent on the addition of
feeder leukocytes plus IL 2. In the latter cases, feeder leukocytes alone,
releasing some IL 2, stimulated growth suboptimally at different cell
concentrations. Their stimulative effect was significantly enhanced when
leukocyte-derived IL 2 or pure recombinant IL 2 was supplemented. Alone, IL
2 (up to 500 U/mL) did not support colony formation. Apparently, IL 2 and
feeder leukocytes are both required for the induction of colonies in these
cases of ALL. From cell sorting of fluorescent anti-common ALL antigen
(CALLA) stained cells it appeared that colonies descended from cells with
high as well as low or negative CALLA expression. Immunophenotyping
demonstrated the presence of the original leukemia markers on colony cells,
but was not indicative of maturation of ALL toward more differentiated B
cells. We suggest that IL 2 can stimulate the in vitro proliferation of
certain neoplastic B lymphocyte progenitors.
Volume 66,
Issue 3,
pp. 556-561,
09/01/1985
Copyright © 1985 by The American Society of Hematology
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