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In vitro analysis of the homing properties of human lymphocytes:
developmental regulation of functional receptors for high endothelial
venules
ST Jalkanen and EC Butcher
Circulating lymphocytes leave the blood by binding to specialized high
endothelial cells lining postcapillary venules in lymphoid organs or sites
of chronic inflammations, migrating through the vessel wall into the
surrounding tissue. The capacity of lymphocytes to recognize and bind to
high endothelial venules (HEVs) is thus central to the overall process of
lymphocyte traffic and recirculation. We show that viable human lymphocytes
bind selectively to HEVs in frozen sections of normal human lymph nodes,
thus defining a simple in vitro model for the study of human lymphocyte
homing properties. Optimal conditions for the quantitative analysis of
lymphocyte-HEV interaction are described. Furthermore, by using this assay,
we demonstrate that the ability of human lymphocyte populations to bind to
HEVs parallels their presumed migratory status in vivo. Thus, thymocytes
and bone marrow cells, which are sessile in vivo, bind poorly to HEVs in
comparison with mature circulating lymphocytes in peripheral blood or in
peripheral lymphoid tissues. These results indicate that HEV-binding
ability is a regulated property of mature lymphocytes and, as demonstrated
previously in animal models, probably plays a fundamental role in
controlling lymphocyte traffic in humans. The in vitro model of
lymphocyte-HEV interaction thus provides a unique means to assay the
migratory properties of normal and neoplastic human lymphocyte subsets, to
analyze the role of lymphocyte traffic mechanisms in normal and pathologic
inflammatory reactions, and to define some of the molecular mechanisms
responsible for the control of lymphocyte migration and positioning in
humans.
Volume 66,
Issue 3,
pp. 577-582,
09/01/1985
Copyright © 1985 by The American Society of Hematology
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