Antibody against T lymphoblastic leukemia-associated antigen (3-40)
identifies vimentin and keratin intermediate filaments in normal cells
AJ Laster, TJ Palker, EA Harden, W Ho, K Naito, B DuPont and BF Haynes
Antibody 3-40 defines a 35- to 40-kd surface antigen present on T
lymphoblastic leukemia (T-ALL) cells that is absent on normal hematopoietic
cells (Naito et al, Blood 62:852, 1983). Using immunoblot analysis of
cytoskeletal proteins and indirect immunofluorescence of cell lines treated
with various cytoskeletal inhibitors, in this report we have demonstrated
that antibody 3-40 also identifies vimentin intermediate filaments (IMF)
within the HSB-2 (T-ALL) cell line as well as in normal thymocytes,
peripheral blood mononuclear cells, and human and rodent fibroblast cell
lines. Cross-reactivity with several keratin subclasses was demonstrated in
both human and rodent epithelial cell lines, human thymus, and skin. In
addition, we have shown that antibody 3-40 defined a 39-kd intracellular
IMF-associated protein in HSB-2 cells, epithelial and fibroblast cell
lines. This IMF-associated protein may be selectively expressed on the
surface of human T cells during malignant transformation.
Volume 66,
Issue 3,
pp. 642-648,
09/01/1985
Copyright © 1985 by The American Society of Hematology
