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NA Cunningham, AP Zola, HL Hui, LM Taylor and FA Green
The relation of human erythrocyte Rh0(D) to Du sites is an important
unresolved question in the field of immunohematology. To compare the
immunological reactivity of Rh0(D)-positive and Du erythrocytes, the
binding characteristics of two anti-Rh0(D) antisera to human Rh0(D)-
positive and Du ("low-grade") erythrocytes were studied. 14C-Protein A and
direct antibody-labeled techniques were used to generate binding curves and
to derive double-reciprocal plots. The results show that the number of
antigen sites differ by a factor of 10 to 15 between the Rh0(D)-positive
and Du red cells, but that the dissociation constants between anti-Rh0(D)
and the Rh0(D) and Du antigens are indistinguishable when studied by the
two labeling methods and two different anti-Rh0(D) antibodies. The extent
of binding to 112 different Du samples showed a normal distribution and was
independent of apparent phenotype. These data suggest immunologic identity
of Rh0(D) and Du ("low-grade") sites and that the difference between the
antigens of Rh0(D) and Du cells is quantitative only. The data are
incompatible with the "missing mosaic" and gene interaction theories of
mechanism.
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| Copyright © 1985 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
