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JD Griffin, KD Sabbath, F Herrmann, P Larcom, K Nichols, M Kornacki, H Levine and SA Cannistra
Expression of HLA-DR surface antigens by granulocyte/monocyte colony-
forming cells (CFU-GM) may be important in the regulation of proliferation
of these cells. Using immunological techniques to enrich for progenitor
cells, we investigated the expression of HLA-DR in subsets of CFU-GM.
"Early" (day 14) CFU-GM express higher levels of HLA- DR than do "late"
(day 7) CFU-GM. Among late CFU-GM, cells destined to form monocyte
(alpha-naphthyl acetate esterase-positive) colonies express higher levels
of HLA-DR than do CFU-GM destined to form granulocyte (chloroacetate
esterase-positive) colonies. Because high- level expression of DR antigen
was a marker for monocyte differentiation, we examined several lymphokines
for their effects on both DR expression and in vitro commitment to monocyte
differentiation by myeloid precursor cells. DR antigen density could be
increased by more than twofold over 48 hours upon exposure to
gamma-interferon (gamma-IFN), whereas colony-stimulating factors had no
effect. This was associated with a dose-dependent inhibition of total
CFU-GM number, and a relative, but not absolute, increase in the ratio of
monocyte colonies to granulocyte colonies. Similarly, in day 7 suspension
cultures of purified myeloid precursor cells, gamma-IFN inhibited cell
proliferation and increased the ratio of monocytes to granulocytes. Thus,
despite the induction of high levels of HLA-DR antigen on precursor cells
(a marker of monocyte commitment), the dominant in vitro effect of
gamma-IFN was inhibition of granulocyte differentiation.
This article has been cited by other articles:
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| Copyright © 1985 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
