Stimulation of bone marrow-derived and peritoneal macrophages by a T
lymphocyte-derived hemopoietic growth factor, persisting cell- stimulating
factor
RM Crapper, G Vairo, JA Hamilton, I Clark-Lewis and JW Schrader
Several lines of evidence indicated that P cell-stimulating factor (PSF), a
T lymphocyte-derived lymphokine known to stimulate the growth of
hemopoietic stem and progenitor cells, also acted on macrophages. PSF was
absorbed from medium that had been mixed for two hours at 0 degrees C with
either resident or thioglycollate-elicited peritoneal cells, suggesting the
presence of receptors for PSF on cells in the population. The addition of
pure PSF to populations highly enriched in either resident or elicited
adherent peritoneal macrophages resulted in stimulation of macrophages with
morphological changes, including increases in size, spreading, vacuolation,
and the number of cytoplasmic processes, together with stimulation of
proliferation and the phagocytosis of opsonized yeast. PSF also stimulated
the incorporation of [3H]thymidine by bone marrow-derived adherent
macrophages. Addition of pure PSF to cultures that contained only a single
macrophage resulted in enhanced survival and proliferation of these
isolated cells, demonstrating that the effect of PSF on macrophages was
direct. These results indicate that PSF can stimulate well-differentiated
functional macrophages and raise the possibility that the effects of PSF on
macrophages may play a regulatory role in immune responses.
Volume 66,
Issue 4,
pp. 859-865,
10/01/1985
Copyright © 1985 by The American Society of Hematology
